![]() Despite extensive literature describing various MS sample preparation methods, there is little standardization among methods, resulting in confusion for those who are new to MS sample preparation techniques, and high variability in MS analysis results, even among expert MS sample prep labs. For MS-based proteomics to reach its full potential as a routinely used detection technology in research and clinical settings, the variability associated with the sample preparation steps that precede MS analysis must be addressed. The quality and consistency of sample preparation influences the time and cost of MS analysis and the reliability and accuracy of results. In order to identify thousands of proteins from a complex lysate, it is essential to have robust sample preparation methods for protein extraction, reduction, alkylation, digestion, and clean-up. Shotgun proteomics is a commonly used strategy to identify proteins in complex mixtures by digesting proteins at specific amino acids into peptides that can be separated and identified by mass spectrometry (Ref.2). ![]() Mass spectrometry (MS) has become a prominent technique in biological research for the identification, characterization, and quantification of proteins (Ref. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |